BS EN 12393-2:2013
Current
The latest, up-to-date edition.
Foods of plant origin. Multiresidue methods for the determination of pesticide residues by GC or LC-MS/MS Methods for extraction and clean-up
Hardcopy , PDF
English
30-11-2013
Foreword
Introduction
1 Scope
2 Normative references
3 Principles
4 General: Summary of procedures
5 Method M: Extraction with acetone and
liquid-liquid partition with dichloromethane/light
petroleum, if necessary clean-up on Florisil[R]
6 Method N: Extraction with acetone, liquid-liquid
partition with dichloromethane or cyclohexane/ethyl
acetate, clean-up with gel permeation and silica gel
chromatography
7 Method P: Extraction with ethyl acetate, and if
necessary, clean-up by gel permeation chromatography
Annex A (informative) - Average water content of
crops and foods
Bibliography
Describes methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues.
| Committee |
AW/275
|
| DevelopmentNote |
Supersedes 96/501164 DC. (08/2005) Supersedes 07/30160744 DC. (12/2008) Supersedes 12/30256808 DC. (11/2013)
|
| DocumentType |
Standard
|
| Pages |
46
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This European Standard specifies methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues.
Different solvents can be used for this purpose. These pesticide residues are generally associated with other co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed, several techniques can be used.
This European Standard contains the following extraction and clean-up methods that have been subjected to interlaboratory studies and/or are adopted throughout Europe:
method M: Extraction with acetone and liquid-liquid partition with dichloromethane/light petroleum, if necessary clean-up on Florisil® 1) [1], [2], [3];
method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane/ethyl acetate and clean-up with gel permeation and silica gel chromatography [4], [5];
method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography [6].
This European Standard specifies the details of methods M, N and P for the extraction and the clean-up of food samples of plant origin. Several solvents at different volumes are used for extraction. Techniques of clean-up are listed such as liquid-liquid partition, liquid chromatography on various adsorbents and gel permeation chromatography.
A table providing the couples (matrix/pesticide) which have been submitted to collaborative studies and a list of indicative applicability of the method to different pesticides are given for each method, wherever possible.
| Standards | Relationship |
| NF EN 12393-2 : 2013 | Identical |
| NEN EN 12393-2 : 2013 | Identical |
| I.S. EN 12393-2:2013 | Identical |
| DIN EN 12393-2:2014-03 | Identical |
| NS EN 12393-2 : 2013 | Identical |
| NBN EN 12393-2 : 2014 | Identical |
| EN 12393-2:2013 | Identical |
| UNE-EN 12393-2:2014 | Identical |
| EN 12393-3:2013 | Foods of plant origin - Multiresidue methods for the determination of pesticide residues by GC or LC-MS/MS - Part 3: Determination and confirmatory tests |
| EN 12393-1:2013 | Foods of plant origin - Multiresidue methods for the determination of pesticide residues by GC or LC-MS/MS - Part 1: General considerations |
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