BS EN 12823-1:2014
Current
The latest, up-to-date edition.
Foodstuffs. Determination of vitamin A by high performance liquid chromatography Measurement of all-E-retinol and 13-Z-retinol
Hardcopy , PDF
English
31-05-2014
Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Examples of HPLC
chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Alternative HPLC systems
Bibliography
Defines a method for determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC).
| Committee |
AW/275
|
| DevelopmentNote |
Supersedes 97/707078 DC. (05/2005) Supersedes 12/30258758 DC. (06/2014)
|
| DocumentType |
Standard
|
| Pages |
20
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This European Standard specifies a method for the determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC). This method has been validated in an interlaboratory study with samples of margarine and milk powder with all-E-retinol levels ranging from 653 µg/100 g to 729 µg/100 g and with 13-Z-retinol levels ranging from 30 µg/100 g to 39 µg/100 g. The determination of vitamin A content is carried out by the measurement of all-E-retinol, 13-Z-retinol and β-carotene. This part covers the measurement of all-E-retinol and 13-Z-retinol.
The extract obtained after saponification in this method can be used for the determination of β-carotene, as described in EN 12823-2:2000, Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of β-carotene. In this case, the saponification temperature should preferably not exceed 80 °C in order to prevent isomerisation and oxidation of β-carotene.
| Standards | Relationship |
| EN 12823-1:2014 | Identical |
| DIN EN 12823-1:2014-08 | Identical |
| NBN EN 12823-1 : 2014 | Identical |
| I.S. EN 12823-1:2014 | Identical |
| NS EN 12823-1 : 2014 | Identical |
| NF EN 12823-1 : 2014 | Identical |
| NEN EN 12823-1 : 2014 | Identical |
| DIN EN 12823-1:2000-07 | Identical |
| UNE-EN 12823-1:2014 | Identical |
| ISO/IEC 17025:2005 | General requirements for the competence of testing and calibration laboratories |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
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