BS EN 16162:2012
Current
The latest, up-to-date edition.
Animal feeding stuffs. Determination of decoquinate by HPLC with fluorescence detection
Hardcopy , PDF
English
31-03-2012
Foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Sample preparation
8 Procedure
9 Calculations
10 Precision
11 Test report
Annex A (informative) - Results inter-laboratory study
Annex B (informative) - Additional recovery results
Annex C (informative) - Additional results for
robustness purposes
Annex D (informative) - HPLC parameters and
chromatogram examples
Bibliography
Describes a method for the determination of decoquinate and high-performance liquid chromatographic (HPLC) method with a fluorescence detection is applicable to the quantification of decoquinate content in complete and complementary compound feeds, medicated feeds, semi-liquid feeds, premixtures and feed additives.
| Committee |
AW/10
|
| DevelopmentNote |
Supersedes 10/30199746 DC. (04/2012)
|
| DocumentType |
Standard
|
| Pages |
28
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This European Standard specifies a method for the determination of decoquinate. This high-performance liquid chromatographic (HPLC) method with a fluorescence detection is applicable to the quantification of decoquinate content in complete and complementary compound feeds, medicated feeds, semi-liquid feeds, premixtures and feed additives.
The method was fully validated from LOQ to 60 000 mg/kg on different matrices during an international collaborative study [11], especially on complete compound feeds for poultry, at trace contamination level of 3 mg/kg and at European authorized level of 20 mg/kg to 40 mg/kg [12].
The limit of detection is between 0,1 mg/kg and 0,3 mg/kg and the limit of quantification is around 0,5 mg/kg. These limits were validated during the collaborative study [11], from results on the blank feed. Lower limits of detection or quantification could be reached but a single laboratory validation is then requested.
| Standards | Relationship |
| EN 16162:2012 | Identical |
| DIN EN 16162:2012-06 | Equivalent |
| I.S. EN 16162:2012 | Equivalent |
| UNE-EN 16162:2012 | Equivalent |
| ISO 6497:2002 | Animal feeding stuffs — Sampling |
| PREN ISO 6498 : DRAFT 2009 | ANIMAL FEEDING STUFFS - GUIDELINES FOR SAMPLE PREPARATION |
| EN ISO 6497:2005 | Animal feeding stuffs - Sampling (ISO 6497:2002) |
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