DD ISO/TS 23893-2:2007
Current
The latest, up-to-date edition.
Water quality. Biochemical and physiological measurements on fish Determination of ethoxyresorufin-O-deethylase (EROD)
Hardcopy , PDF
English
31-03-2008
Foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 Test environment
5 Reagents
6 Apparatus
7 Sampling and preparation of samples
7.1 Sampling of fish
7.2 Killing of fish and dissection
7.3 Storage
8 Procedure
8.1 Preparation of the fractions
8.2 Determination of protein
8.3 Determination of the EROD activity
8.4 Checking the sensitivity of the biological reagent and
compliance with the operating method
9 Expression of results
9.1 Calculation of the EROD activity
9.2 Statistical processing of the data
10 Test report
Annex A (informative) - Examples of results
Bibliography
Defines a method for measuring the ethoxyresorufin-O-deethylase (EROD) enzyme activity on a post-mitochondrial fraction of fish liver homogenate (subcellular fraction in which the EROD activity is located) employing a cell or microplate fluorimetric method.
| Committee |
EH/3/5
|
| DevelopmentNote |
Supersedes 06/30085853 DC. (03/2008)
|
| DocumentType |
Standard
|
| Pages |
26
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This part of ISO23893 specifies a method for measuring the ethoxyresorufin-O-deethylase (EROD) enzyme activity on a post-mitochondrial fraction of fish liver homogenate (subcellular fraction in which the EROD activity is located) employing a cell or microplate fluorimetric method.
It applies to fish that are sampled in their natural environment (fresh water or salt water) or exposed to substances or effluents in a laboratory.
This method is applicable for EROD values greater than or equal to 1pmol/(min·mg) of proteins. A higher sensitivity may be achieved by using a cell (test tube) procedure.
| Standards | Relationship |
| ISO/TS 23893-2:2007 | Identical |
| ISO 23893-1:2007 | Water quality — Biochemical and physiological measurements on fish — Part 1: Sampling of fish, handling and preservation of samples |
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