EN 12822:2014
Current
The latest, up-to-date edition.
Foodstuffs - Determination of vitamin E by high performance liquid chromatography - Measurement of α-, β-, γ- and δ-tocopherol
04-06-2014
Foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Procedure
8 Calculation
9 Precision
10 Test report
Annex A (informative) - Examples of HPLC
chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Alternative HPLC systems
Bibliography
This European Standard specifies a method for the determination of vitamin E in foods by high performance liquid chromatography (HPLC). The determination of vitamin E content is carried out by measurement of α-, β , γ- and δ-tocopherol. This method has been validated in two interlaboratory studies. The first study was for the analysis of α-tocopherol in margarine and milk powder ranging from 9,89 mg/100 g to 24,09 mg/100 g. The second study was for the analysis of α-, β-, γ- and δ-tocopherol in milk powder and of α-, and β-tocopherol in oat powder ranging from 0,057 mg/100 g (β-tocopherol) to 10,2 mg/100 g (α-tocopherol).NOTEThe vitamin E activity can be calculated from the tocopherol content assuming appropriate factors as given in [1], [2], [3] and [4].
| Committee |
CEN/TC 275
|
| DevelopmentNote |
Supersedes PREN 12822. (06/2014)
|
| DocumentType |
Standard
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Current
|
| Supersedes |
| Standards | Relationship |
| NF EN 12822 : 2014 | Identical |
| NBN EN 12822 : 2014 | Identical |
| NEN EN 12822 : 2014 | Identical |
| NS EN 12822 : 2014 | Identical |
| I.S. EN 12822:2014 | Identical |
| PN EN 12822 : 2014 | Identical |
| BS EN 12822:2014 | Identical |
| DIN EN 12822:2014-08 | Identical |
| UNE-EN 12822:2014 | Identical |
| DIN EN 12822:2000-07 | Identical |
| S.R. CWA 16814:2014 | NUTRITIONALLY CORRECT LOW-COST FOOD FOR PEOPLE AT RISK OF POVERTY - GENERAL, SPECIFIC REQUIREMENTS AND LABELLING OF CHANCE FOOD |
| 15/30324769 DC : 0 | BS ISO 20633 - INFANT FORMULA AND ADULT NUTRITIONALS - DETERMINATION OF VITAMIN E AND VITAMIN A BY NORMAL PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
| ISO 20633:2015 | Infant formula and adult nutritionals — Determination of vitamin E and vitamin A by normal phase high performance liquid chromatography |
| BS ISO 20633:2015 | Infant formula and adult nutritionals. Determination of vitamin E and vitamin A by normal phase high performance liquid chromatography |
| ISO/IEC 17025:2005 | General requirements for the competence of testing and calibration laboratories |
| ISO 5725:1986 | Precision of test methods — Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |