EN 15607:2009
Current
The latest, up-to-date edition.
Foodstuffs - Determination of d-biotin by HPLC
27-05-2009
Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Typical chromatogram
Annex B (informative) - Precision data
Bibliography
This European Standard specifies a method for the determination of the mass fraction of d-biotin by high performance liquid chromatography (HPLC). The method has been validated in an inter-laboratory test on fortified and non-fortifed samples such as cereal breakfast powder, infant milk powder, lyophilized green peas with ham, lyophilized chicken soup and on nutritive orange juice, at levels from 16 μg/100 g to 200 μg/100 g. For further information on the validation data, see Annex B. NOTE 1 d-biocytin can also be estimated by this method. But none of the samples used for the validation step contained d-biocytin. Nonetheless the recovery rate is more than 90 % for d-biotin and d-biocytin, see [2] and [3]. NOTE 2 The method underestimates the real biotin content when used for samples containing egg.
| Committee |
CEN/TC 275
|
| DocumentType |
Standard
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Current
|
| Standards | Relationship |
| I.S. EN 15607:2009 | Identical |
| DIN EN 15607:2009-09 | Identical |
| NEN EN 15607 : 2009 | Identical |
| NS EN 15607 : 2009 | Identical |
| NF EN 15607 : 2009 | Identical |
| NBN EN 15607 : 2009 | Identical |
| BS EN 15607:2009 | Identical |
| UNE-EN 15607:2010 | Identical |
| 04/30125241 DC : DRAFT NOV 2004 | EN 15085-4 - RAILWAY APPLICATIONS - WELDING OF RAILWAY VEHICLES AND COMPONENTS - PART 4: PRODUCTION REQUIREMENTS |
| NF EN 15001-1 : 2009 | GAS INFRASTRUCTURE - GAS INSTALLATION PIPEWORK WITH AN OPERATING PRESSURE GREATER THAN 0,5 BAR FOR INDUSTRIAL INSTALLATIONS AND GREATER THAN 5 BAR FOR INDUSTRIAL AND NON-INDUSTRIAL INSTALLATIONS - PART 1: DETAILED FUNCTIONAL REQUIREMENTS FOR DESIGN, MATERIALS, CONSTRUCTION, INSPECTION AND TESTING |
| ISO 5725-2:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |