EN 16159:2012

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus and equipment
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Results of the inter-laboratory
        test
Annex B (informative) - Flowchart - Determination of
        selenium by hydride generation atomic
        absorption spectrometry (HGAAS) after
        microwave digestion (digestion with 65%
        nitric acid and 30% hydrogen peroxide
Annex C (informative) - Alternative digestion procedure
        with the same digestion efficiency: Acid
        digestion with a mixture of 65% nitric acid
        and 70% perchloric acid (7:3 by volume)
        at atmospheric pressure
Bibliography

This European Standard specifies a method for the determination of selenium in animal feeding stuffs by hydride generation atomic absorption spectrometry (HGAAS) after microwave pressure digestion. The method was successfully tested by an inter-laboratory study of CEN/TC 327/WG 4 in the range of 0,25 mg/kg to 74 mg/kg.The limit of quantification is 0,5 µg/l of the test solution which corresponds to the calibration standard 2. Using a test portion of 0,5 g and a volume of the test solution of 25 ml after pressure digestion the limit of quantification is calculated as 0,125 mg/kg in the feed material.NOTEA lower limit of quantification could be achieved – each laboratory has to prove it.