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I.S. EN 16418:2014

Current

Current

The latest, up-to-date edition.

PAPER AND BOARD - DETERMINATION OF THE CYTOTOXICITY OF AQUEOUS EXTRACTS USING A METABOLICALLY COMPETENT HEPATOMA CELL LINE (HEPG[2])

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-01-2014

Preview

For Harmonized Standards, check the EU site to confirm that the Standard is cited in the Official Journal.

Only cited Standards give presumption of conformance to New Approach Directives/Regulations.

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Cell line
7 Food simulants used for testing
8 Cleaning laboratory glassware
9 Equipment
10 Preparation of specimens
11 Cytotoxicity assessment
12 Expression of the results
13 Interpretation of the results
14 Precision
15 Test report
Annex A (informative) - 96-well plates configuration
Annex B (informative) - RNA Synthesis rate
        inhibition cytotoxicity test work flow
Annex C (informative) - Validation of the two
        methods (option A and B)
Bibliography

Defines a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG2 cell line.

DocumentType
Standard
Pages
24
PublisherName
National Standards Authority of Ireland
Status
Current

Standards Relationship
EN 16418:2014 Identical

EN 645:1993 Paper and board intended to come into contact with foodstuffs - Preparation of a cold water extract
EN 15845:2010 Paper and board - Determination of the cytotoxicity of aqueous extracts
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
EN 647:1993 Paper and board intended to come into contact with foodstuffs - Preparation of a hot water extract

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