PD ISO/TS 19006:2016
Current
The latest, up-to-date edition.
Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
Hardcopy , PDF
English
31-08-2016
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
5 Materials
6 Technical equipment
7 Nanoparticle sample preparation
8 Preparations
9 Evaluation of nanoparticle impact on ROS generation in cells
10 Data analysis and results
Annex A (informative) - Alternate cell lines
Annex B (informative) - Alternate fluorescence characterization
techniques
Annex C (informative) - Suspension preparation and characterization
Annex D (informative) - Example experimental data from RAW 264.7
Bibliography
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