VDI 4331 Blatt 2:2013-08
Current
The latest, up-to-date edition.
Monitoring the effects of genetically modified organisms (GMOs) - Method for extracting nucleic acids from soil for the analysis of microbial communities and detection of transgenic DNA - Quality requirements and applications
Hardcopy , PDF
German - English
01-08-2013
Preliminary note
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Quality requirements for nucleic
acid extracts
5 List of materials
6 Procedure
Annex A - Protocol for DNA extraction
from soil samples
Annex B - Special modification of a
Protocol for DNA extraction
from soils with a higher amount
of humic material
Annex C - Protocol for simultaneous
extraction of DNA and RNA
from soil samples
Bibliography
Nucleic acids extracted from soil can serve as a starting material for the detection of microorganisms living in soil and for assessing their diversity.
| DevelopmentNote |
Supersedes VDI 4331-7. (08/2013)
|
| DocumentType |
Standard
|
| Pages |
27
|
| PublisherName |
Verlag des Vereins Deutscher Ingenieure
|
| Status |
Current
|
| Supersedes |
| VDI 4331 Blatt 1:2014-01 | Monitoring the effects of genetically modified organisms - Effects on soil organisms |
| VDI 4330 Blatt 5:2015-11 | Monitoring the effects of genetically modified organisms (GMOs) - Guidelines for the collection and preparation of plant samples for molecular biological analysis |
| VDI 4331 Blatt 1:2014-01 | Monitoring the effects of genetically modified organisms - Effects on soil organisms |
| ISO 10381-1:2002 | Soil quality — Sampling — Part 1: Guidance on the design of sampling programmes |
| ISO 10381-6:2009 | Soil quality — Sampling — Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory |
| ISO 24276:2006 | Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — General requirements and definitions |
| VDI 4330 Blatt 1:2006-10 | Monitoring the ecological effects of genetically modified organisms - Genetically modified plants - Basic principles and strategies |
| ISO 21571:2005 | Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Nucleic acid extraction |
| VDI 1000:2017-02 | VDI Standardisation Work - Principles and procedures |
| DIN EN ISO 21571:2013-08 | FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION (ISO 21571:2005 + AMD 1:2013) |
| VDI 4330 Blatt 7:2006-12 | Monitoring the effects of genetically modified organisms (GMO) - Qualitative methods for the detection of genetically engineered nucleic acids in the environment |
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