AS 60068.2.10-2003

1 - AS 60068.2.10-2003 ENVIRONMENTAL TESTING - TESTS-TEST J AND GUIDANCE: MOULD GROWTH
4 - PREFACE
5 - CONTENTS
7 - 1 General
7 - 2 Health hazards to operators
8 - 3 Scope
8 - 4 Reagents and materials
8 - 4.1 Cultures or spores – Supply and condition
9 - 4.2 Preparation of mould suspensions
10 - 4.3 Control strips
10 - 5 Description of test apparatus
10 - 5.1 For small specimens
11 - 5.2 For large specimens
11 - 6 Severities
11 - 7 Initial examination
11 - 8 Preconditioning
11 - 8.1 For test variants 1 and 2
11 - 8.2 For test variant 2
12 - 9 Conditioning
12 - 9.1 Application
12 - 9.1.1 Variant 1
12 - 9.2 Inoculation
12 - 9.3 Incubation
13 - 10 Final examination
13 - 10.1 Visual examination
13 - 10.2 Effect of growth
13 - 10.3 Extent of growth
14 - 11 Information to be given in the relevant specification
15 - Annex A - Danger to personnel
15 - A.1 General
15 - A.2 Notes for the guidance of medical officers
17 - Annex B - Inoculation methods
18 - Annex C - Recommended safety precautions
20 - Annex D - Decontamination procedures
21 - Annex E - Flow-chart
22 - Annex F - Guidance
22 - F.1 Mechanisms of contamination
22 - F.2 Germination and growth
23 - F.3 Effects of growth
24 - F.4 Prevention of mould growth
25 - F.5 Applicability of the mould growth test
26 - F.6 Danger to personnel and recommended safety precautions
26 - F.7 Use of fungicides

This test covers the inoculation of assembled specimens with a selection of mould spores followed by a period of incubation under conditions which promote spore germination and the growth of mould.

To investigate unforeseen causes of deterioration in assembled specimens, whether or not constructed from mould-resistant materials by the application of test variant 1 and/or test variant 2 using the required severity given in the relevant specification. a) Test variant 1: by assessing the extent of mould growth after 28 days' incubation and any physical damage caused thereby, and if required by the relevant specification, by checking the effect on functioning of the specimen after incubation extended to a total of 84 days. b) Test variant 2: by assessing the extent of mould growth after 28 days' incubation following quasi-contamination with nutrients and any physical damage caused thereby, and by checking the effect on the functioning of the specimen. The relevant specification shall state the required test variant and severity.

First published as part of AS C333-1963.
AS C333-1963 revised and rdesignated in part as AS 1099.2J-1971.
AS 1099.2J-1971 revised and rdesignated as AS 1099.2.10-1989.
AS 1099.2.10-1989 revised and rdesignated as AS 60068.2.10-2003.