AS/NZS 4276.17.2:2000

1 - AS/NZS 4276.17.2:2000 WATER MICROBIOLOGY - SPORES OF SULFITE-REDUCING ANAEROBES (CLOSTRIDIA) INCLUDING CLOSTRIDIUM PERFRINGEN
1 - PREFACE
2 - METHOD
2 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 PRINCIPLE
2 - 4 MEDIA, REAGENTS, AND REFERENCE CULTURES
2 - 4.1 Culture media (see AS 4276.2 and Appendix B)
2 - 4.2 Nitrite detection reagents (see Appendix B)
2 - 4.3 Reference culture
3 - 5 APPARATUS
3 - 5.1 Suitable screw cap containers for the volume of water to be tested
3 - 5.2 Pipettes or equivalent measuring devices
3 - 5.3 Incubators and water baths
3 - 6 TEST PROCEDURES
3 - 6.1 MPN by multiple tube dilution technique
4 - 7 CONFIRMATION OF C.perfringens
4 - 8 REPORT
5 - 9 BIBLIOGRAPHY
6 - APPENDIX A - FLOW CHART OF MPN EXAMINATION PROCEDURES FOR SPORES OF SULFITE- REDUCING CLOSTRIDIA AND CLOSTRIDIUM PERFRINGENS
7 - APPENDIX B - CULTURE MEDIA
7 - B1 SCOPE
7 - B2 DIFFERENTIAL REINFORCED CLOSTRIDIAL MEDIUM (single strength)
7 - B3 TRYPTOSE SULFITE CYCLOSERINE AGAR
8 - B4 LACTOSE GELATIN MEDIUM
8 - B5 NITRATE MOTILITY MEDIUM
8 - B6 NITRITE DETECTION REAGENTS
8 - B6.1 Solution A, Sulfanilic acid reagent
9 - B6.2 Solution B, N-( 1- naphthyl) ethylenediamine reagent

This Standard sets out a method for determining the most probable number (MPN) of spores of sulfite-reducing anaerobes (clostridia) including C.perfringens in water, using a multiple tube dilution technique, with a confirmation procedure for identifying C.perfringens.

This Standard sets out a method for determining the most probable number (MPN) of sporesof sulfite-reducing anaerobes (clostridia) including C.perfringens in water, using a multipletube dilution technique, with a confirmation procedure for identifying C.perfringens.Refer to AS 4276.1 for a description of the general procedure for estimating the MPN ofmicroorganisms.MPN procedures may be used for the examination of all water samples and are particularlyapplicable for samples containing sufficient suspended material to make filtrationprocedures impractical.NOTE: A flow diagram of the procedure is shown in Appendix A.

First published as AS/NZS 4276.17.2:2000.