BS 6068-5.4(1985) : 1985

National foreword
Method
0 Introduction
1 Scope and field of application
2 Principle
3 Test organism and reagents
4 Apparatus
5 Test environment
6 Procedure
7 Expression of results
8 Test report
9 Bibliography
Annexes
A Environmental parameters for maintenance and breeding
   of zebra fish (Brachydanio rerio Hamilton-Buchanan)
B Suggested form for recording data
Figures
1 Example of apparatus for flow-through method
2 Graphical interpolation of LC 50 (linear scales)
3 Graphical interpolation of LC 50 (logarithmic and
   probability scales)

Specifies a flow-through method for the determination of the acute lethal toxicity of substances soluble in water under specified conditions to a species of freshwater fish (common name, zebra fish) in water of a specified quality. Also applies when using certain other species of freshwater fish as the test organism. Coverage includes apparatus, procedure and test environment. Also includes annexes and diagrams.