Milk. Enumeration of somatic cells Microscopic method (Reference method)

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

11-30-2009

Publisher

British Standards Institution

Foreword
Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Reagents
   4.1 Dye solutions
   4.2 Phosphate Buffer Solution (PBS)
5 Apparatus
6 Sampling
7 Preparation of test sample
   7.1 Storage
   7.2 Preparation
8 Procedure
   8.1 Preparation of the smear and staining
   8.2 Determination
9 Calculation and expression of results
   9.1 Rectangular shape counting in successive fields
   9.2 Rectangular shape counting in bands
   9.3 Circular shape counting in successive fields
   9.4 Expression of results
10 Precision
   10.1 Repeatability
   10.2 Reproducibility
11 Test report
Annex A (informative) - Collaborative trial
Annex B (informative) - Staining for goats' milk
Annex C (informative) - Poisson distribution
Bibliography

Describes a microscopic method (reference method) for the counting of somatic cells in both raw and chemically preserved milk.

Committee	AW/5
DevelopmentNote	Supersedes 95/501531 DC. (11/2005) Supersedes 06/30151198 DC. (03/2008)
DocumentType	Standard
Pages	28
PublisherName	British Standards Institution
Status	Current
Supersedes	06/30151198 DC : DRAFT JUN 2006 95/501531 DC : JUN 95 BS EN ISO 13366-1:1997

Standards	Relationship
ISO 13366-1:2008/Cor 1:2009	Identical
NS EN ISO 13366-1 : 2008 AC 2009	Identical
NEN EN ISO 13366-1 : 2008 C1 2009	Identical
NF EN ISO 13366-1 : 2008	Identical
ISO 13366-1:2008	Identical
SN EN ISO 13366-1 : 2008 CORR 2010	Identical
NBN EN ISO 13366-1 : 2008 COR 2009	Identical
I.S. EN ISO 13366-1:2008	Identical
EN ISO 13366-1:2008/AC:2009	Identical
UNI EN ISO 13366-1 : 2008	Identical
UNE-EN ISO 13366-1:2008	Identical
DIN EN ISO 13366-1:2008-06	Identical

ISO 5725-1:1994	Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 707:2008	Milk and milk products — Guidance on sampling
ISO 13366-2:2006	Milk Enumeration of somatic cells Part 2: Guidance on the operation of fluoro-opto-electronic counters
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method

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BS EN ISO 13366-1:2008

Milk. Enumeration of somatic cells Microscopic method (Reference method)

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Abstract

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