BS ISO 7251:2005
Withdrawn
Microbiology of food and animal feeding stuffs. Horizontal method for the detection and enumeration of presumptive Escherichia coli. Most probable number technique
Hardcopy , PDF
English
05-05-2005
10-20-2023
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Detection method
4.2 Enumeration method
5 Diluent, culture media and reagent
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Detection method
9.2 Enumeration method
10 Expression of results
10.1 Detection method
10.2 Enumeration method
11 Test report
Annex A (normative) MPN table
Bibliography
Provides general guidelines for the detection and enumeration of presumptive Escherichia coli by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37 degree C, then at 44 degree C.
| Committee |
AW/9
|
| DevelopmentNote |
Supersedes BS 5763-8(1994). (05/2005)
|
| DocumentType |
Standard
|
| Pages |
22
|
| PublisherName |
British Standards Institution
|
| Status |
Withdrawn
|
| Supersedes |
This International Standard gives general guidelines for the detection and enumeration of presumptive Escherichia coli by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37°C, then at 44°C. This International Standard is applicable to
-
products intended for human consumption and the feeding of animals, and
-
environmental samples in the area of food production and food handling.
WARNING — Some pathogenic strains of Escherichia coli do not grow at 44°C.
A limitation of the applicability of this International Standard is imposed by the susceptibility of the method to a large degree of variability (see Clause10).
| Standards | Relationship |
| ISO 7251:2005 | Identical |
| ISO/TS 11133-1:2009 | Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory |
| ISO 6887-1:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO/TS 11133-2:2003 | Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 2: Practical guidelines on performance testing of culture media |
| ISO 8261:2001 | Milk and milk products — General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination |
| ISO 6887-4:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of miscellaneous products |
| ISO 6887-3:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products |
| ISO 6887-2:2017 | Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and meat products |
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