EN ISO 20837:2006
Withdrawn
Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection (ISO 20837:2006)
04-15-2006
10-31-2006
Foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 General laboratory requirements
5 Reagents, apparatus and equipment
6 Procedure
Annex A (informative) Standards concerning the enrichment of
microorganisms (bacteria)
Annex B (informative) Method for DNA extraction from
Gram-negative bacteria
Bibliography
ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B. ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.
| Committee |
CEN/TC 463
|
| DocumentType |
Standard
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Withdrawn
|
| Standards | Relationship |
| ISO 20837:2006 | Identical |
| NS EN ISO 20837 : 1ED 2006 | Identical |
| BS EN ISO 20837:2006 | Identical |
| NBN EN ISO 20837 : 2006 | Identical |
| I.S. EN ISO 20837:2006 | Identical |
| SS-EN ISO 20837 : 2006 | Identical |
| NEN EN ISO 20837 : 2006 | Identical |
| PN EN ISO 20837 : 2007 | Identical |
| ONORM EN ISO 20837 : 2006 | Identical |
| UNE-EN ISO 20837:2007 | Identical |
| UNE-EN ISO 20837:2006 | Identical |
| DIN EN ISO 20837:2006-08 | Identical |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 6579:2002 | Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp. |
| ISO 21567:2004 | Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Shigella spp. |
| ISO 11290-1:2017 | Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 1: Detection method |
| ISO/TS 20836:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Performance testing for thermal cyclers |
| ISO 7937:2004 | Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of Clostridium perfringens — Colony-count technique |
| ISO 10272-1:2017 | Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method |
| ISO 6888-3:2003 | Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 3: Detection and MPN technique for low numbers |
| ISO 7932:2004 | Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of presumptive Bacillus cereus — Colony-count technique at 30 degrees C |
| ISO 10273:2017 | Microbiology of the food chain — Horizontal method for the detection of pathogenic Yersinia enterocolitica |
| ISO 20838:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods |
| ISO 16654:2001 | Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157 |
| DIN 10135:2013-05 | MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - METHOD FOR THE DETECTION OF SALMONELLA |