ISO/TS 21569-2:2012
Withdrawn
View Superseded by
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products
Hardcopy , PDF
English, Russian
09-03-2012
04-09-2025
This method describes a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as "CDC Triffid"). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopalin synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.
| Committee |
ISO/TC 34/SC 16
|
| DocumentType |
Technical Specification
|
| Pages |
9
|
| PublisherName |
International Organization for Standardization
|
| Status |
Withdrawn
|
| SupersededBy |
| Standards | Relationship |
| PD ISO/TS 21569-2:2012 | Identical |
| NEN NPR ISO/TS 21569-2 : 2012 | Identical |
| ISO/IEC 17025:2005 | General requirements for the competence of testing and calibration laboratories |
| ISO 24276:2006 | Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — General requirements and definitions |
| ISO 21571:2005 | Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Nucleic acid extraction |
| ISO 21569:2005 | Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Qualitative nucleic acid based methods |
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