S.R. CEN ISO/TS 15216-2:2013
Withdrawn
View Superseded by
MICROBIOLOGY OF FOOD AND ANIMAL FEED - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS IN FOOD USING REAL-TIME RT-PCR - PART 2: METHOD FOR QUALITATIVE DETECTION (ISO/TS 15216-2:2013, CORRECTED VERSION 2013-05-01)
Hardcopy , PDF
English
01-01-2013
03-31-2020
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and materials
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Test report
Annex A (normative) - Diagram of procedure
Annex B (informative) - Real-time RT-PCR mastermixes
and cycling parameters
Annex C (informative) - Real-time RT-PCR primers and
hydrolysis probes for the detection of HAV, norovirus
GI and GII and mengo virus (process control)
Annex D (informative) - Growth of mengo virus strain MC[0]
for use as a process control
Annex E (informative) - RNA extraction using the BioMerieux
NucliSens[R] system
Annex F (normative) - Composition and preparation of reagents
and buffers
Annex G (informative) - Generation of external control RNA
(EC RNA) stocks
Annex H (informative) - Typical optical plate layout
Bibliography
Defines a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces.
| DocumentType |
Standard
|
| Pages |
43
|
| PublisherName |
National Standards Authority of Ireland
|
| Status |
Withdrawn
|
| SupersededBy |
| Standards | Relationship |
| ISO/TS 15216-2:2013 | Identical |
| CEN ISO/TS 15216-2:2013 | Identical |
| UNE-CEN ISO/TS 15216-2:2013 | Equivalent |
| PD CEN ISO/TS 15216-2:2013 | Equivalent |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 22118:2011 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics |
| ISO 22119:2011 | Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
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