BS EN ISO 14939:2001
Current
The latest, up-to-date edition.
Animal feeding stuffs. Determination of carbadox content. Method using high performance liquid chromatography
Hardcopy , PDF
English
18-10-2001
1 Scope
2 Normative reference
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparation of test sample
8 Procedure
8.1 General
8.2 Preparation of spiked sample
8.3 Extraction
8.4 Column chromatography
8.5 HPLC analysis
9 Confirmation
9.1 General
9.2 Co-chromatography
9.3 Diode array detector
9.4 Post-column derivatization
10 Calculation of results
10.1 General
10.2 Feeding stuffs containing 0,1 mg/kg to 10 mg/kg
of carbadox
10.3 Feeding stuffs containing 0,1 mg/kg to 100 mg/kg
of carbadox
10.4 Premixtures containing up to 10 % of carbadox
11 Precision
11.1 Interlaboratory test
11.2 Repeatability
11.3 Reproducibility
12 Test report
Annex A (informative) Flow chart
Annex B (informative) Results of interlaboratory test
Bibliography
Describes a high-performance liquid chromatographic (HPLC) method for the determination of the carbadox content in premixtures and animal feeding stuffs. This method is applicable to animal feeding stuffs with a mass fraction of carbadox of 0,5 mg/kg (limit of quantification) to 100 mg/kg, and to premixtures with a mass fraction of carbadox up to 10 %. The lower limit of detection is 0,1 mg/kg.
| Committee |
AW/10
|
| DevelopmentNote |
Supersedes 99/703133 DC (11/2001)
|
| DocumentType |
Standard
|
| Pages |
26
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This International Standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the carbadox content in premixtures and animal feeding stuffs.
The method is applicable to animal feeding stuffs with a mass fraction of carbadox of 0,5mg/kg (limit of quantification) to 100mg/kg, and to premixtures with a mass fraction of carbadox up to 10%.
The lower limit of detection is 0,1mg/kg.
NOTE1 For animal feeding stuffs the mass fraction of carbadox is expressed in milligrams per kilogram, and for premixtures as a percentage by mass.
NOTE2 Carbadox is a chemotherapeuticum belonging to the quinoxaline group. Carbadox is used as a growth-promoting feed additive for piglets.
| Standards | Relationship |
| NEN EN ISO 14939 : 2001 | Identical |
| I.S. EN ISO 14939:2002 | Identical |
| NBN EN ISO 14939 : 2001 | Identical |
| EN ISO 14939:2001 | Identical |
| NS EN ISO 14939 : 1ED 2001 | Identical |
| ISO 14939:2001 | Identical |
| NF EN ISO 14939 : 2002 | Identical |
| DIN EN ISO 14939:2001-12 | Equivalent |
| DIN EN ISO 14939:2001-12 | Identical |
| UNE-EN ISO 14939:2002 | Identical |
| ISO 5725-1:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions |
| ISO 5725-2:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method |
| ISO 6498:2012 | Animal feeding stuffs — Guidelines for sample preparation |
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