EN ISO 14939:2001

Foreword
1 Scope
2 Normative reference
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparation of test sample
8 Procedure
   8.1 General
   8.2 Preparation of spiked sample
   8.3 Extraction
   8.4 Column chromatography
   8.5 HPLC analysis
9 Confirmation
   9.1 General
   9.2 Co-chromatography
   9.3 Diode array detector
   9.4 Post-column derivatization
10 Calculation of results
   10.1 General
   10.2 Feeding stuffs containing 0,1 mg/kg to 10 mg/kg
        of carbadox
   10.3 Feeding stuffs containing 0,1 mg/kg to 100 mg/kg
   10.4 Premixtures containing up to 10 % of carbadox
11 Precision
   11.1 Interlaboratory test
   11.2 Repeatability
   11.3 Reproducibility
12 Test report
Annex A (informative) Flow chart
Annex B (informative) Results of interlaboratory test
Bibliography

This International Standard specifies a high-performance liquid chromatographic (HPLC) method for thedetermination of the carbadox content in premixtures and animal feeding stuffs.The method is applicable to animal feeding stuffs with a mass fraction of carbadox of 0,5 mg/kg (limit ofquantification) to 100 mg/kg, and to premixtures with a mass fraction of carbadox up to 10 %.The lower limit of detection is 0,1 mg/kg.NOTE 1 For animal feeding stuffs the mass fraction of carbadox is expressed in milligrams per kilogram, and for premixturesas a percentage by mass.NOTE 2 Carbadox is a chemotherapeuticum belonging to the quinoxaline group. Carbadox is used as a growth-promotingfeed additive for piglets.