EN 17280:2019

This document specifies a procedure for the determination of nivalenol (NIV), deoxynivalenol (DON) and its acetyl derivatives (3-acetyl-DON and 15-acetyl-DON), HT-2 and T-2 toxins (HT-2 and T-2) and zearalenone (ZEN) in cereals and cereal products by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) after clean-up by solid phase extraction (SPE).The method has been validated with samples of wheat, wheat flour, and wheat crackers. The wheat and the wheat flour was prepared from a mixture of wheat and fungi infected wheat kernels. The wheat crackers were baked from wheat flour and water spiked with the target mycotoxins.Validation levels for NIV ranged from 27,7 μg/kg to 378 μg/kg.Validation levels for DON ranged from 234 μg/kg to 2420 μg/kg.Validation levels for 3-acetyl-DON ranged from 18,5 μg/kg to 137 μg/kg.Validation levels for 15-acetyl-DON ranged from 11,4 μg/kg to 142 μg/kg.Validation levels for HT-2 ranged from 6,6 μg/kg to 134 μg/kg.Validation levels for T-2 ranged from 2,1 μg/kg to 37,6 μg/kg.Validation levels for ZEN ranged from 31,6 μg/kg to 230 μg/kg.Laboratory experiences have shown that this method is also applicable to barley and oat flour, and rye based crackers [5], however, this has not been validated in a collaborative study.