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MILK AND MILK-BASED DRINKS - DETERMINATION OF ALKALINE PHOSPHATASE ACTIVITY - ENZYMATIC PHOTO-ACTIVATED SYSTEM (EPAS) METHOD

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-01-2007

Publisher

National Standards Authority of Ireland

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For Harmonized Standards, check the EU site to confirm that the Standard is cited in the Official Journal.

Only cited Standards give presumption of conformance to New Approach Directives/Regulations.

Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparations
   7.1 Test samples
   7.2 Alkaline phosphatase-free milk
8 Procedure
   8.1 Calibration
   8.2 Calibration adjustment
   8.3 Control tests and calibration check
   8.4 Determination
   8.5 Heat-resistant microbial alkaline phosphatase
        control
9 Calculation and expression of results
   9.1 General
   9.2 Manual calculation
   9.3 Expression of results
10 Precision
   10.1 Interlaboratory test
   10.2 Repeatability
   10.3 Reproducibility
11 Test report
Annex A (informative) Procedure flowchart
Annex B (informative) Results of interlaboratory
        trials
Bibliography

Defines a method for the determination of the alkaline phosphatase activity in pasteurized whole milk, semi-skimmed milk, skimmed milk, cream and flavoured milks using a chemiluminescent (EPAS) method.

DocumentType	Standard
Pages	28
PublisherName	National Standards Authority of Ireland
Status	Current
Supersedes	I.S. EN ISO 11816-1:2000

Standards	Relationship
UNE-EN ISO 22160:2007	Identical
DIN EN ISO 22160:2007-08	Identical
BS EN ISO 22160:2007	Identical
NEN EN ISO 22160 : 2007	Identical
UNI EN ISO 22160 : 2007	Identical
ISO 22160:2007	Identical
EN ISO 22160:2007	Identical
NS EN ISO 22160 : 1ED 2007	Identical
NBN EN ISO 22160 : 2007	Identical
NF EN ISO 22160 : 2007	Identical
SN EN ISO 22160 : 2007	Identical

ISO 5725-1:1994	Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 11816-1:2013	Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks
ISO 11816-2:2016	Milk and milk products — Determination of alkaline phosphatase activity — Part 2: Fluorimetric method for cheese
ISO 707:2008	Milk and milk products — Guidance on sampling
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method

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I.S. EN ISO 22160:2007

MILK AND MILK-BASED DRINKS - DETERMINATION OF ALKALINE PHOSPHATASE ACTIVITY - ENZYMATIC PHOTO-ACTIVATED SYSTEM (EPAS) METHOD

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