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BS ISO 13322-1:2014



The latest, up-to-date edition.

Particle size analysis. Image analysis methods Static image analysis methods

Available format(s)

Hardcopy , PDF



Published date


1 Scope
2 Normative references
3 Terms and definitions and list of symbols
4 Preparation for image capture
5 Sample preparation demands for method description
6 Quality of captured images
7 Image analysis
8 Counting procedure
9 Calculation of the particle size results
10 Calibration and traceability
11 Accuracy
12 Test report
Annex A (informative) - Estimation of the number of
        particles to be counted for a given accuracy
Annex B (informative) - Common segmentation
        methods for particle edge detection
Annex C (informative) - Flow chart showing a typical
        image analysis method

Pertains to the analysis of images for the purpose of determining particle size distributions where the velocity of the particles against the axis of the optical system of the imaging device is zero.

This part of ISO13322 is applicable to the analysis of images for the purpose of determining particle size distributions where the velocity of the particles against the axis of the optical system of the imaging device is zero. The particles are appropriately dispersed and fixed in the object plane of the instrument. The field of view may sample the object plane dynamically either by moving the sample support or the camera provided this can be accomplished without any motion effects on the image. Captured images can be analysed subsequently.

This part of ISO13322 concentrates upon the analysis of digital images created from either light or electron detection systems. It does not address the method of creating the image although the detection settings chosen together with its calibration are important to particle sizing accuracy. This part of ISO13322 considers only image evaluation methods using complete pixel counts.

Both the type of distribution, (by number or by volume) together with the width of the particle size distribution has a very material influence upon the number of particles to be measured to secure the desired accuracy within the specified confidence limits. An example is shown in AnnexA.

Automation of the analysis is possible in order to measure sufficient particle numbers for a required degree of precision.

This part of ISO13322 does not address the sample preparation. However, the sub sampling, dispersion and presentation of particles to be measured are a vital part of the operational chain of actions necessary to ensure accuracy and precision of any final result.

NOTE Further details about sampling and sample preparation can be found in ISO14887 and ISO14488.

Supersedes 03/314397 DC. (02/2006) Supersedes 11/30232144 DC. (05/2014)
British Standards Institution

Standards Relationship
ISO 13322-1:2014 Identical

PD 6699-1:2007 Nanotechnologies Good practice guide for specifying manufactured nanomaterials

ISO 9276-1:1998 Representation of results of particle size analysis — Part 1: Graphical representation
ISO 14887:2000 Sample preparation Dispersing procedures for powders in liquids
ISO Guide 34:2009 General requirements for the competence of reference material producers
ISO 9276-2:2014 Representation of results of particle size analysis Part 2: Calculation of average particle sizes/diameters and moments from particle size distributions
ISO 14488:2007 Particulate materials — Sampling and sample splitting for the determination of particulate properties

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