01/707544 DC : DRAFT JUNE 2001
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
PREN 14105 - FAT AND OIL DERIVATIVES - FATTY ACID METHYL ESTERS (FAME) - DETERMINATION OF FREE AND TOTAL GLYCEROL AND MONO-, DI-, TRIGLYCERIDE CONTENTS - REFERENCE METHOD
15-05-2003
23-11-2012
Foreword
1 Scope
2 Principle
3 Reagents
4 Apparatus
5 Procedure
5.1 Preparation and analysis of the calibration solutions
5.2 Preparation and analysis of the commercial mixture of
monoglycerides
5.3 Preparation and analysis of the samples
5.4 Identification
5.5 Calibration
6 Expression of results
6.1 Integration of the peaks
6.2 Glycerol calibration function
6.3 Glycerides calibration function
6.4 Calculation of the percentage of free glycerol
6.5 Calculation of the percentage of glycerides
6.6 Calculation of the percentage of total glycerol
6.7 Expression of results
7 Precision
7.1 Repeatability
7.2 Reproducibility
8 Test report
Annex A Calibration function calculation - Linear regression
method
Annex B Worked example
Annex C (informative) Chromatogram of a rapeseed FAME
example - determination of mono-, di-, triglycerides
and free glycerol
Annex D (informative) Results of an interlaboratory trial
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