15/30311173 DC : 0
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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BS EN ISO 15216-1 - MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS IN FOOD USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION
Hardcopy , PDF
English
30-04-2017
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and materials
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Precision
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (informative) - Real-time RT-PCR
mastermixes and cycling parameters
Annex C (informative) - Real-time RT-PCR primers
and hydrolysis probes for the detection of
HAV, norovirus GI and GII and mengo
virus (process control)
Annex D (informative) - Growth of mengo virus
strain MC[0] for use as a process control
Annex E (informative) - RNA extraction using
the BioMerieux NucliSens[R] system
Annex F (normative) - Composition and preparation
of reagents and buffers
Annex G (informative) - Generation of double-stranded
DNA (dsDNA) control stocks
Annex H (informative) - Generation of external control
RNA (EC RNA) stocks
Annex I (informative) - Typical optical plate layout
Annex J (informative) - Results of interlaboratory study
Bibliography
BS EN ISO 15216-1.
| Committee |
AW/9
|
| DocumentType |
Draft
|
| Pages |
54
|
| PublisherName |
British Standards Institution
|
| Status |
Superseded
|
| SupersededBy |
| ISO 22174:2005 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
| ISO 7218:2007 | Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
| ISO 22118:2011 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics |
| ISO 22119:2011 | Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
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