AS/NZS 1766.2.15:1998

1 - AS/NZS 1766.2.15:1998 FOOD MICROBIOLOGY - EXAMINATION FOR SPECIFIC ORGANISMS-LISTERIA MONOCYTOGENES IN DAIRY PRODUCTS
1 - PREFACE
2 - CONTENTS
3 - 1 Scope
3 - 2 Normative references
3 - 3 Definitions
3 - 3.1 Listeria spp.:
3 - 3.2 detection of Listeria monocytogenes:
3 - 4 Principle
3 - 4.1 Enrichment in selective liquid medium
4 - 4.2 Isolation and presumptive identification
4 - 4.3 Confirmation
4 - 5 Culture media and reagent
4 - 5.1 General
4 - 5.2 Culture media
4 - 5.2.1 Selective medium: Enrichment medium
5 - 5.2.2 Isolation medium (Oxford agar)
5 - 5.2.3 Solid cul ture medium: Tryptone soya yeast extract agar (TSYEA)
5 - 5.2.4 Liquid culture medium: Tryptone soya yeast extract broth (TSYEB)
6 - 5.2.5 Blood agar
6 - 5.2.6 Carbohydrate utilization broth
6 - 5.2.7 Motility medium
6 - 5.2.8 CAMP (Christie, Atkins, Munch-Petersen) test
7 - 5.3 Reagent
7 - 6 Apparatus and glassware
7 - 6.1 Apparatus
7 - 6.1.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave) (see ISO 7218)
7 - 6.1.2 Incubator,
7 - 6.1.3 Incubator,
7 - 6.1.4 Incubator,
7 - 6.1.5 Waterbaths,
7 - 6.1.6 Blending equipment
8 - 6.1.7 Loops,
8 - 6.1.8 Inoculating needle,
8 - 6.1.9 Temperature-compensated pH-meter
8 - 6.1.10 Refrigerator
8 - 6.1.11 Source of beamed whi te light
8 - 6.1.12 Mirror,
8 - 6.1.13 Tripod,
8 - 6.1.14 Phase-contrast microscope,
8 - 6.2 Glassware
8 - 6.2.1 Culture flasks,
8 - 6.2.2 Culture tubes
8 - 6.2.3 Measuring cylinders,
8 - 6.2.4 Graduated pipettes,
8 - 6.2.5 Sterile Petri dishes
8 - 6.2.6 Microscopic slides
8 - 6.2.7 Glass beads
8 - 7 Sampling
8 - 8 Preparation of test sample
8 - 8.1 Milk
8 - 8.2 Dried milk, dried whey, dried buttermilk, lactose, case in and caseinate
8 - 8.3 Butter
8 - 8.4 Cheese
8 - 8.5 Edible ices
8 - 8.6 Fermented milks, yoghurts, custards and desserts
9 - 9 Procedure
9 - 9.1 Inoculation of enrichment medium
9 - 9.1.1 Milk
9 - 9.1.2 Dried milk, dried whey, dried buttermilk and caseinate
9 - 9.1.3 Casein
9 - 9.1.4 Butter
9 - 9.1.5 Cheese
9 - 9.1.6 Frozen milk products (including edible ices)
9 - 9.1.7 Fermented milks, yoghurts, custards and desserts
9 - 9.2 Incubation
9 - 9.3 Isolation and presumptive identification
9 - 9.4 Confirmation
9 - 9.4.1 Selection of colonies for confirmation
9 - 9.4.2 Incubation
10 - 9.4.3 Catalase reaction
10 - 9.4.4 Morphology and staining properties
10 - 9.4.5 Haemol ysis
11 - 9.4.6 Further biochemical confirmation
11 - 9.5 Interpretation of morphological and physiological properties and of biochemical reaction (see table 1)
12 - 9.6 Definitive confirmation
12 - 9.7 Control cultures
12 - 10 Expression of results
12 - 11 Test report
13 - Annex A - SAFETY PRECAUTIONS
14 - Annex B - BIBLIOGRAPHY
15 - TECHNICAL CORRIGENDUM 1
16 - ANNEX ZZ - VARIATIONS TO ISO 10560:1993 FOR AUSTRALIAN/NEW ZEALAND CONDITIONS
16 - ZZ1 SCOPE
16 - ZZ2 VARIATIONS

The objective of this Standard is to specify a method for the detection of Listeria monocytogenes in milk and milk products.

This InternationalStandard specifies methods for the detection of Listeria monocytogenes in milk and milk products.

First published in Australia as AS 1766.2.15(Int)-1991.
Withdrawn 1993.
Jointly revised and designated AS/NZS 1766.2.15:1998.