EN 15911:2010

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus and equipment
6 Procedure
7 Calculation of results
8 Precision
9 Test report
Annex A (informative) - Table A.1 - Suitable method conditions
Annex B (informative) - Examples of chromatograms
Annex C (informative) - Precision data
Annex D (informative) - Present EU limits for the nine sweeteners
Bibliography

This European Standard specifies a method for the simultaneous determination of nine sweeteners in beverages and canned or bottled fruits by high performance liquid chromatography (HPLC) with evaporative light scattering detection (HPLC-ELSD). This method has been validated in an interlaboratory study via the analysis of spiked samples on the following matrices:-acesulfame-K (ACS-K) (from 38,3 mg/l to 383,5 mg/l) in beverages and (from 38,4 mg/kg to 391,3 mg/kg) in canned fruits;-alitame (ALI) (from 31,1 mg/l to 114,5 mg/l) in beverages and (from 36 mg/kg to 175,2 mg/kg) in canned fruits;-aspartame (ASP) (from 38,1 mg/l to 702 mg/l) in beverages and (from 37,2 mg/kg to 1 120,2 mg/kg) in canned fruits;-cyclamic acid (CYC) (from 28,3 mg/l to 307,2 mg/l) in beverages and (from 27,5 mg/kg to 1 100,6 mg/kg) in canned fruits;-dulcin (DUL) (from 55,0 mg/l to 115,1 mg/l) in beverages and (from 49,8 mg/kg to 172,6 mg/kg) in canned fruits;-neotame (NEO) (from 37,6 mg/l to 115,3 mg/l) in beverages and (from 37,3 mg/kg to 173,7 mg/kg) in canned fruits;-neohesperidine dihydrochalcone (NHDC) (from 31,4 mg/l to 59,3 mg/l) in beverages and (from 35,3 mg/kg to 59,3 mg/kg) in canned fruits;-saccharin (SAC) (from 36,2 mg/l to 87,6 mg/l) in beverages and (from 44,3 mg/kg to 235,3 mg/kg) in canned fruits;-sucralose (SCL) (from 36,8 mg/l to 346,8 mg/l) in beverages and (from 35,3 mg/kg to 462,4 mg/kg) in canned fruits.For further information on the validation results see Annex C.NOTEThe method has been fully validated [1] through collaborative trial, according to the IUPAC Harmonised Protocol [2], on analyte-matrix combinations of all nine sweeteners in beverages and canned or bottled fruits.