EN ISO 17375:2006

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedures
  6.1 Conditioning of IACs
  6.2 Extraction
  6.3 Immunoaffinity clean up
  6.4 Post-column derivatization
  6.5 Calibration curve
  6.6 Calculation
  6.7 Spiking procedures for recovery determination
7 Precision
  7.1 Interlaboratory test
  7.2 Repeatability
  7.3 Reproducibility
8 Test report
Annex A (informative) Results of an interlaboratory test
Bibliography

ISO 17375:2006 specifies a method for the determination of aflatoxin B1 in animal feeding stuffs using high-performance liquid chromatography with post-column derivatization.It is applicable to animal feeding stuffs with a fat content of up to 50 %.The limit of quantification of this method has been demonstrated to be better than 0,5 g/kg for aflatoxin B1 for a signal-to-noise ratio of 6.