UNE-CEN ISO/TS 21569-10:2026
Current
The latest, up-to-date edition.
Horizontal methods for molecular biomarker analysis - Methods of analysis for the detection of genetically modified organisms and derived products - Part 10: Construct- and event-specific detection methods for genetically modified salmon expressing CS-GHc2 growth hormone (ISO/TS 21569 10:2026) (Endorsed by Asociación Española de Normalización in July of 2026.)
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English
01-07-2026
This document specifies procedures for the detection of a DNA sequence of a construct used to (genetically) enhance the growth of fish commonly found in aquaculture. The genetically modified AquAdvantage Atlanticsalmon (Salmo salar) carries the construct expressing CS-GHc2 growth hormone and can be detected based on a real-time polymerase chain reaction (PCR) targeting either the border between the growth hormonecoding sequence (CS-GHc2) of Oncorhynchus tshawytscha (Chinook salmon) and the antifreeze terminator (T-AFP) of (Macro-) Zoarces americanus (ocean pout), i.e. with the construct-specific method, or the borderbetween the Atlantic salmon genomic DNA and the antifreeze promoter (P-AFP) of ocean pout, i.e. with the event-specific method. These methods can be applied to identify the genetically modified (GM) fish or forscreening purposes.This document is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs. The application of these methods requiresthe extraction of an adequate amount of amplifiable DNA from the relevant matrix.
| Committee |
CTN 34/SC 4
|
| DocumentType |
Standard
|
| Pages |
15
|
| PublisherName |
Asociación Española de Normalización
|
| Status |
Current
|
| Standards | Relationship |
| CEN ISO/TS 21569-10:2026 | Identical |
| PD CEN ISO/TS 21569-10:2026 | Equivalent |
| ISO/TS 21569-10:2026 | Identical |
| NSAI/CEN ISO/TS 21569-10:2026 | Equivalent |
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