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ASTM D 7847 : 2017

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by

Standard Guide for Interlaboratory Studies for Microbiological Test Methods

Available format(s)

Hardcopy , PDF

Superseded date

23-08-2022

Language(s)

English

Published date

28-06-2017

1.1 Microbiological test methods present challenges that are unique relative to chemical or physical parameters, because microbes proliferate, die off and continue to be metabolically active in samples after those samples have been drawn from their source.

Committee
D 02
DocumentType
Guide
Pages
9
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

1.1Microbiological test methods present challenges that are unique relative to chemical or physical parameters, because microbes proliferate, die off and continue to be metabolically active in samples after those samples have been drawn from their source.

1.1.1Microbial activity depends on the presence of available water. Consequently, the detection and quantification of microbial contamination in fuels and lubricants is made more complicated by the general absence of available water from these fluids.

1.1.2Detectability depends on the physiological state and taxonomic profile of microbes in samples. These two parameters are affected by various factors that are discussed in this guide, and contribute to microbial data variability.

1.2This guide addresses the unique considerations that must be accounted for in the design and execution of interlaboratory studies intended to determine the precision of microbiological test methods designed to quantify microbial contamination in fuels, lubricants and similar low water-content (water activity <0.8) fluids.

1.3This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

1.4This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D 2777 : 2021 Standard Practice for Determination of Precision and Bias of Applicable Test Methods of Committee D19 on Water
ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration

ASTM D 4175 : 2020 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2014 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2009 : REV A : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM E 1326 : 1998 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM D 6300 : 2020 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2008 : REV B : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 6469 : 2004 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 6300 : 2013 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM E 1326 : 2015 Standard Guide for Evaluating Non-culture Microbiological Tests Used for Enumerating Bacteria
ASTM D 6974 : 2016 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
ASTM D 4175 : 2005 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4012 : 1981 : R2009 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM D 7463 : 2016 : EDT 1 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
ASTM D 6300 : 2014 : REV A : EDT 1 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 7463 : 2014 : REV A Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
ASTM D 7464 : 2020 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
ASTM D 4175 : 2005 : EDT 2 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4012 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM E 1326 : 2015 : REV A Standard Guide for Evaluating Non-culture Microbiological Tests
ASTM D 1129 : 2003 Standard Terminology Relating to Water
ASTM D 4175 : 2015 : REV C Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 6974 : 2009 : R2013 : EDT 2 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
ASTM D 4175 : 2020 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2017 : REV A : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6300 : 2017 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 1129 : 2013 Standard Terminology Relating to Water
ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
ASTM D 7463 : 2021 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
ASTM D 6300 : 2017 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 4175 : 2008 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2020 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4012 : 1981 : R2002 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM D 4175 : 2017 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2009 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM E 1326 : 2006 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM D 6469 : 2012 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 8070 : 2020 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
ASTM D 4175 : 2022 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6300 : 2016 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 4175 : 2009 : REV A : EDT 4 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2009 : REV A : EDT 3 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 6469 : 2008 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 6300 : 2004 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 1129 : 2006 Standard Terminology Relating to Water
ASTM D 7463 : 2008 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water
ASTM D 4175 : 2018 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6974 : 2009 : R2013 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
ASTM D 7978 : 2014 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method
ASTM D 6300 : 2019 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM E 1326 : 2008 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM D 4175 : 2001 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2020 : REV B : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2001 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2005 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 7464 : 2019 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
ASTM D 7978 : 2014 : R2019 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method
ASTM D 7463 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
ASTM D 6300 : 2021 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6300 : 2019 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 4175 : 2002 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM E 1259 : 2010 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
ASTM E 2756 : 2019 Standard Terminology Relating to Antimicrobial and Antiviral Agents
ASTM D 6300 : 2020 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 7687 : 2011 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel, Fuel/Water Mixtures, and Fuel-Associated Water with Sample Concentration by Filtration
ASTM D 8070 : 2019 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
ASTM D 7464 : 2008 : R2013 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
ASTM D 4175 : 2015 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM E 1601 : 2019 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
ASTM D 4175 : 2002 : REV A : EDT 2 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 7687 : 2021 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
ASTM D 6469 : 2014 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
ASTM D 1129 : 2002 Standard Terminology Relating to Water
ASTM E 1601 : 1998 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
ASTM D 6300 : 2020 : REV B Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6300 : 2006 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM E 2756 : 2010 Standard Terminology Relating to Antimicrobial and Antiviral Agents
ASTM E 1259 : 1994 Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
ASTM E 1601 : 2010 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
ASTM D 7463 : 2018 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
ASTM D 4175 : 2009 : REV A : EDT 5 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2016 : REV C Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2015 : REV B Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 8070 : 2021 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
ASTM D 6300 : 2014 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 6300 : 2015 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 4175 : 2015 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 6300 : 1999 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 4175 : 2009 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2016 : REV B Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2002 : REV A : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2008 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM E 1601 : 2012 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
ASTM D 6974 : 2009 : R2013 : EDT 1 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
ASTM D 4175 : 2002 : REV A Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 1129 : 2004 Standard Terminology Relating to Water
ASTM D 6974 : 2009 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
ASTM D 4012 : 1981 : R1997 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM D 4175 : 2000 : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 4175 : 2016 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6300 : 2013 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 7464 : 2014 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
ASTM D 4175 : 2019 : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 7463 : 2016 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
ASTM D 6974 : 2003 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels-Filtration and Culture Procedures
ASTM D 6974 : 2020 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
ASTM E 1259 : 2005 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
ASTM D 8070 : 2016 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
ASTM D 6300 : 2008 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 6300 : 2003 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 6469 : 2011 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
ASTM D 6300 : 2007 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM E 1259 : 2001 Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
ASTM E 2756 : 2018 Standard Terminology Relating to Antimicrobial and Antiviral Agents
ASTM D 6469 : 1999 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 4175 : 2017 : REV A Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6300 : 2002 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 4175 : 2009 : REV A : EDT 2 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM D 6469 : 2008 : EDT 1 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 4175 : 2018 : EDT 1 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2016 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6300 : 2016 : REV A Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM D 6974 : 2004 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
ASTM D 7463 :2014 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water
ASTM D 1129 : 2010 Standard Terminology Relating to Water
ASTM E 1259 : 2018 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
ASTM D 1129 : 2001 Standard Terminology Relating to Water
ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
ASTM D 4175 : 2017 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 4175 : 2019 Standard Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants
ASTM D 6300 : 2014 Standard Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants
ASTM E 1259 : 2016 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C
ASTM D 4175 : 2015 : REV B : EDT 1 Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants
ASTM E 1326 : 2013 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM D 7464 : 2008 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
ASTM D 4175 : 2008 : REV B Standard Terminology Relating to Petroleum, Petroleum Products, and Lubricants

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