BS EN 12822:2014
Current
The latest, up-to-date edition.
Foodstuffs. Determination of vitamin E by high performance liquid chromatography. Measurement of α-, β-, γ- and δ-tocopherol
Hardcopy , PDF
English
30-06-2014
Foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Procedure
8 Calculation
9 Precision
10 Test report
Annex A (informative) - Examples of HPLC
chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Alternative HPLC systems
Bibliography
Describes a method for the determination of Vitamin E in foodstuffs by high performance liquid chromatography (HPLC).
| Committee |
AW/275
|
| DevelopmentNote |
Supersedes 97/707077 DC. (05/2005) Supersedes 12/30258770 DC. (06/2014)
|
| DocumentType |
Standard
|
| Pages |
24
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This European Standard specifies a method for the determination of vitamin E in foods by high performance liquid chromatography (HPLC). The determination of vitamin E content is carried out by measurement of α-, β-, γ- and δ-tocopherol. This method has been validated in two interlaboratory studies. The first study was for the analysis of α-tocopherol in margarine and milk powder ranging from 9,89 mg/100 g to 24,09 mg/100 g. The second study was for the analysis of α-, β-, γ- and δ-tocopherol in milk powder and of α-, and β-tocopherol in oat powder ranging from 0,057 mg/100 g (β-tocopherol) to 10,2 mg/100 g (α-tocopherol).
NOTE The vitamin E activity can be calculated from the tocopherol content assuming appropriate factors as given in [1], [2], [3] and [4].
| Standards | Relationship |
| NBN EN 12822 : 2014 | Identical |
| I.S. EN 12822:2014 | Identical |
| NEN EN 12822 : 2014 | Identical |
| EN 12822:2014 | Identical |
| NF EN 12822 : 2014 | Identical |
| DIN EN 12822:2014-08 | Identical |
| NS EN 12822 : 2014 | Identical |
| UNE-EN 12822:2014 | Identical |
| DIN EN 12822:2000-07 | Identical |
| ISO/IEC 17025:2005 | General requirements for the competence of testing and calibration laboratories |
| ISO 5725:1986 | Precision of test methods — Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
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