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ASTM E 1498 : 1992 : R2012

Withdrawn
Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

Standard Guide for Conducting Sexual Reproduction Tests with Seaweeds (Withdrawn 2021)
Available format(s)

Hardcopy , PDF

Withdrawn date

01-22-2021

Language(s)

English

Published date

01-09-2013

CONTAINED IN VOL. 11.06, 2013 Defines procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water on sexual reproduction by seaweeds.

1.1This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water on sexual reproduction by seaweeds. The exposure duration is species dependent and is followed by a period of development to allow the evidence of sexual reproduction to appear. There is no exposure to toxicants during the development period. This restricts the tests primarily to the events surrounding egg fertilization, and it minimizes any timelag effects on development that might interfere with correct enumeration of the number of sexual events that occurred. These procedures will probably be useful for conducting sexual reproduction toxicity tests with a variety of species of seaweeds, although modifications might be necessary.

1.2Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparison of the results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sexual reproduction tests with seaweeds.

1.3These procedures are applicable to most chemicals, either individually or in formulations, commercial products, and known mixtures or whole effluents, as well as for use in testing surface waters. With appropriate modifications, these procedures can be used to study the effects of temperature, dissolved oxygen, pH, and such materials as leachates, oils, particulate matter, and sediments.

1.4The values stated in SI units are to be regarded as the standard.

1.5This guide is arranged as follows:

Section

Referenced Documents

2 

Terminology

3 

Summary of Guide

4 

Significance and Use

5 

Apparatus

6 

 Facilities

6.1

 Construction Materials

6.2

 Test Chambers

6.3

 Cleaning

6.4

 Acceptability

6.5

Hazards

7 

Dilution and Culture Water

8 

 Requirements

8.1

 Source and Treatment

8.2

Test Material

9 

 Single Chemicals

9.1

 Stock Solutions

9.2

 Effluents and Surface Waters

9.3

 Test Concentrations

9.4

Test Organism

10 

 Species

10.1

 Life Stage

10.2

 Source

10.3

 Culture Nutrient Medium

10.4

Procedure

11 

 Preparation of Plants for a Test

11.1

 Test Conditions

11.2

 Experimental Design

11.3

Acceptability of Test

12 

Calculation of Results

13 

Documentation

14 

Keywords

15 

Appendix

X1


1.6This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazards statements, see 6.4 and Section 7.

Committee
E 50
DocumentType
Guide
Pages
12
ProductNote
Reconfirmed 2012
PublisherName
American Society for Testing and Materials
Status
Withdrawn
Supersedes

ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests

ASTM E 1023 : 1984 : R2007 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 380 : 1993 Standard Practice for Use of the Internation System of Units (SI) (the Modernized Metric System) (Withdrawn 1997)
ASTM E 1023 : 1984 : R2014 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1023 : 1984 : R2002 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1192 : 1997 : R2008 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1023 : 1984 : R1996 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1192 : 1997 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1192 : 1997 : R2003 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1192 : 1997 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

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