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ASTM E 729 : 1996

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Available format(s)

Hardcopy , PDF

Superseded date

11-11-2014

Language(s)

English

Published date

05-10-1996

CONTAINED IN VOL. 11.06, 2014 Specifies procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water, but not to food, on certain species of freshwater and saltwater fishes, macroinvertebrates, and amphibians during 2 to 8-day exposures, depending on the species.

1.1 This guide (1) describes procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water, but not to food, on certain species of freshwater and saltwater fishes, macroinvertebrates, and amphibians during 2- to 8-day exposures, depending on the species, using the static, renewal, and flow-through techniques. These procedures will probably be useful for conducting acute toxicity tests with many other aquatic species, although modifications might be necessary.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute tests.

1.3 Selection of the technique to be used in a specific situation will depend upon the needs of the investigator and upon available resources. Static tests provide the most easily obtained measure of acute toxicity, but should not last longer than 96 h. Renewal and flow-through tests may last longer than 96 h because the pH and the concentrations of dissolved oxygen and test material are maintained at desired levels and degradation and metabolic products are removed. Static tests might not be applicable to materials that have a high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed in aqueous solution, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test. Flow-through tests are generally preferable to renewal tests, although in some situations a renewal test might be more cost-effective than a flow-through test. Shell deposition tests with bivalve molluscs must be flow-through tests.

1.4 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications these procedures can be used to conduct acute tests on temperature, dissolved oxygen, pH, and on such materials as leachates, oils, particulate matter, sediments, and surface waters. See Guide E1192 Procedures for conducting acute tests on aqueous effluents.

1.5 In the development of these procedures, an attempt was made to balance scientific and practical considerations and to ensure that the results will be sufficiently accurate and precise for the applications for which they are commonly used. A major consideration was that the common uses of the results of acute toxicity tests do not require or justify stricter requirements than those set forth herein. Although the tests may be improved by using more organisms, longer acclimation times, etc., the requirements presented herein should usually be sufficient.

1.6 Results of acute toxicity tests should usually be reported in terms of an LC50 (median lethal concentration) or EC50 (median effective concentration). In some situations, it might only be necessary to determine whether a specific concentration is acutely toxic to the test species or whether the LC50 or EC50 is above or below a specific concentration.

1.7 This guide is arranged as follows:

Section Referenced Documents 2 Terminology 3 Summary of Standard 4 Significance and Use 5 Hazards 6 Apparatus 7 Facilities 7.1 Special Requirements 7.2 Construction Materials 7.3 Metering System 7.4 Test Chambers 7.5 Cleaning 7.6 Acceptability 7.7 Dilution Water 8 Requirements 8.1 Source 8.2 Treatment 8.3 Characterization 8.4 Test Material 9 General 9.1 Stock Solution 9.2 Test Concentration(s) 9.3 Test Organisms 10 Species 10.1 Age 10.2 Source 10.3 Care and Handling 10.4 Feeding 10.5 Disease Treatment 10.6 Holding 10.7 Acclimation 10.8 Quality 10.9 Procedure 11 Experimental Design 11.1 Dissolved Oxygen 11.2 Temperature 11.3 Loading 11.4 Beginning the Test 11.5 Feeding 11.6 Duration of Test 11.7 Biological Data 11.8 Other Measurements 11.9 Analytical Methodology 12 Acceptability of Test 13 Calculation of Results 14 Report 15

1.8 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.

Committee
E 47
DocumentType
Guide
Pages
22
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy

MIL F 0024385 : E FIRE EXTINGUISHING AGENT, AQUEOUS FILM-FORMING FOAM (AFFF) LIQUID CONCENTRATE, FOR FRESH AND SEA WATER
ASTM E 1367 : 2003 : R2014 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1562 : 2000 : R2013 Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids
MIL F 24385 : F FIRE EXTINGUISHING AGENT, AQUEOUS FILM FORMING FOAM, (AFFF) LIQUID CONCENTRATE, FOR FRESH AND SEA WATER
ASTM E 1023 : 1984 : R2002 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1563 : 1998. Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos
ASTM E 1849 : 1996 Standard Guide for Fish and Wildlife Incident Monitoring and Reporting
ASTM E 1611 : 2000 Standard Guide for Conducting Sediment Toxicity Tests with Marine and Estuarine Polychaetous Annelids
ASTM E 1242 : 1997 Standard Practice for Using Octanol-Water Partition Coefficient to Estimate Median Lethal Concentrations for Fish Due to Narcosis
ASTM E 1415 : 1991 : R2004 Standard Guide for Conducting Static Toxicity Tests With <i>Lemna gibba</i><b> G3</b>
ASTM E 1850 : 2004 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests
ASTM E 1022 : 1994 : R2013 Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks (Withdrawn 2022)
ASTM E 1242 : 1997 : R2008 Standard Practice for Using Octanol-Water Partition Coefficient to Estimate Median Lethal Concentrations for Fish Due to Narcosis
ASTM E 1241 : 2005 Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes
ASTM D 5660 : 1996 Standard Test Method for Assessing the Microbial Detoxification of Chemically Contaminated Water and Soil Using a Toxicity Test with a Luminescent Marine Bacterium
ASTM E 1440 : 1991 : R2004 Standard Guide for Acute Toxicity Test with the Rotifer Brachionus
ASTM D 5951 : 1996 Standard Practice for Preparing Residual Solids Obtained After Biodegradability Standard Methods for Plastics in Solid Waste for Toxicity and Compost Quality Testing
ASTM E 2122 : 2002 Standard Guide for Conducting In-situ Field Bioassays With Caged Bivalves
ASTM E 1498 : 1992 : R1998 Standard Guide for Conducting Sexual Reproduction Tests with Seaweeds
ASTM E 1768 : 1995 : R2003 : EDT 1 Standard Guide for Ventilatory Behavioral Toxicology Testing of Freshwater Fish
ASTM E 1295 : 2001 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <i>Ceriodaphnia dubia</i>
ASTM E 1440 : 1991 : R2012 Standard Guide for Acute Toxicity Test with the Rotifer Brachionus (Withdrawn 2021)
ASTM D 6081 : 1998 Standard Practice for Aquatic Toxicity Testing of Lubricants: Sample Preparation and Results Interpretation
ASTM E 1688:2010 Standard Guide for Determination of the Bioaccumulation of Sediment-Associated Contaminants by Benthic Invertebrates
ASTM E 1023 : 1984 : R2007 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1367 : 2003 : EDT 1 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 2317 : 2004 : R2012 Standard Guide for Conducting Renewal Microplate-Based Life-Cycle Toxicity Tests with a Marine Meiobenthic Copepod (Withdrawn 2021)
ASTM E 1391 : 2003 : R2014 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 2455 : 2006 Standard Guide for Conducting Laboratory Toxicity Tests with Freshwater Mussels
ASTM E 1711 : 2012 : REDLINE Standard Guide for Measurement of Behavior During Fish Toxicity Tests
ASTM E 1415 : 1991 : R1998 Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3
ASTM E 1563 : 1998 : R2012 Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos
ASTM E 1022 : 1994 Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks
NFPA 18A : 2017 WATER ADDITIVES FOR FIRE CONTROL AND VAPOR MITIGATION
NFPA 18 : 2017 WETTING AGENTS
ASTM E 1706 : 2005 : R2010 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates (Withdrawn 2019)
ASTM E 2552 : 2016 : REDLINE Standard Guide for Assessing the Environmental and Human Health Impacts of New Compounds for Military Use
ASTM E 2591 : 2007 Standard Guide for Conducting Whole Sediment Toxicity Tests with Amphibians
ASTM E 1415 : 1991 : R2004 : EDT 1 Standard Guide for Conducting Static Toxicity Tests With <i>Lemna gibba</i> G3
ASTM E 2317 : 2004 Standard Guide for Conducting Renewal Microplate-Based Life-Cycle Toxicity Tests with a Marine Meiobenthic Copepod
ASTM E 1023 : 1984 : R1996 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1562 : 2000 Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids
ASTM E 1924 : 1997 : R2004 Standard Guide for Conducting Toxicity Tests with Bioluminescent Dinoflagellates
ASTM E 1463 : 1992 : R2004 Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States
ASTM E 1193 : 1997 : R2004 Standard Guide for Conducting <I>Daphnia magna</I> Life-Cycle Toxicity Tests
ASTM E 1366 : 2011 : R2016 Standard Practice for Standardized Aquatic Microcosms: Fresh Water
ASTM E 1768 : 1995 Standard Guide for Ventilatory Behavioral Toxicology Testing of Freshwater Fish
ASTM E 2591 : 2007 : R2013 Standard Guide for Conducting Whole Sediment Toxicity Tests with Amphibians
ASTM E 1498 : 1992 : R2012 Standard Guide for Conducting Sexual Reproduction Tests with Seaweeds (Withdrawn 2021)
ASTM E 1391 : 2003 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 1841 : 2004 : R2012 Standard Guide for Conducting Renewal Phytotoxicity Tests With Freshwater Emergent Macrophytes (Withdrawn 2021)
ASTM D 5660 : 1996 : R2004 Standard Test Method for Assessing the Microbial Detoxification of Chemically Contaminated Water and Soil Using a Toxicity Test with a Luminescent Marine Bacterium
ASTM E 724 : 1998 : R2012 Standard Guide for<brk type="line"/> Conducting Static Acute Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs
ASTM E 724 : 1998 : R2004 Standard Guide for Conducting Static Acute Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs
ASTM E 1193 : 1997 : R2012 <i xmlns:a="http://library.astm.org" xmlns="">Daphnia magna</i>
ASTM E 1850 : 2004 : R2012 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests
ASTM E 1440 : 1991 : R1998 Standard Guide for Acute Toxicity Test with the Rotifer Brachionus
ASTM E 1295 : 2001 : R2006 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <i> Ceriodaphnia dubia</i>
ASTM E 724:1998 Standard Guide for Conducting Static Acute Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs
ASTM E 1913 : 2004 Standard Guide for Conducting Static, Axenic, 14-Day Phytotoxicity Tests in Test Tubes with the Submersed Aquatic Macrophyte, <i>Myriophyllum sibiricum Komarov</i>
ASTM E 1023 : 1984 : R2014 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1849 : 1996 : R2013 Standard Guide for Fish and Wildlife Incident Monitoring and Reporting (Withdrawn 2022)
ASTM E 1849 : 1996 : R2007 Standard Guide for Fish and Wildlife Incident Monitoring and Reporting
ASTM E 1525 : 2002 : R2014 Standard Guide for Designing Biological Tests with Sediments
ASTM E 1563 : 1998 : R2004 : EDT 1 Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos
ASTM E 1367 : 2003 : R2008 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1415 : 1991 : R2012 Standard Guide for Conducting Static Toxicity Tests With <i>Lemna gibba</i> G3 (Withdrawn 2021)
ASTM E 1525 : 2002 : R2008 Standard Guide for Designing Biological Tests with Sediments
ASTM E 2122 : 2002 : R2013 Standard Guide for Conducting In-situ Field Bioassays With Caged Bivalves (Withdrawn 2022)
ASTM E 1924 : 1997 Standard Guide for Conducting Toxicity Tests with Bioluminescent Dinoflagellates
ASTM E 1706 : 2005 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 1624 : 1994 : R2008 Standard Guide for Chemical Fate in Site-Specific Sediment/Water Microcosms (Withdrawn 2013)
ASTM E 1367 : 2003 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1192 : 1997 : R2003 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1768 : 1995 : R2013 Standard Guide for Ventilatory Behavioral Toxicology Testing of Freshwater Fish (Withdrawn 2022)
ASTM D 6081 : 1998 : R2014 Standard Practice for Aquatic Toxicity Testing of Lubricants: Sample Preparation and Results Interpretation
ASTM E 1706 : 2005 : EDT 1 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
NFPA 1150 : 2017 FOAM CHEMICALS FOR FIRES IN CLASS A FUELS
ISO 35103:2017 Petroleum and natural gas industries — Arctic operations — Environmental monitoring
ASTM E 1022 : 1994 : R2002 Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks
ASTM E 1366 : 2011 Standard Practice for Standardized Aquatic Microcosms: Fresh Water
ASTM E 1611 : 2000 : R2007 Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids
ASTM E 1849 : 1996 : R2002 Standard Guide for Fish and Wildlife Incident Monitoring and Reporting
ASTM E 1191 : 2003 : REV A Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
ASTM E 1218 : 2004 : R2012 Standard Guide for Conducting Static Toxicity Tests with Microalgae (Withdrawn 2021)
ASTM E 1563 : 1998 : R2004 Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos
ASTM E 1604 : 2012 : REDLINE Standard Guide for Behavioral Testing in Aquatic Toxicology
ASTM E 1191 : 2003 : REV A : R2014 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
ASTM E 1611 : 2000 : R2013 Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids
ASTM D 5660 : 1996 : R2009 Standard Test Method for Assessing the Microbial Detoxification of Chemically Contaminated Water and Soil Using a Toxicity Test with a Luminescent Marine Bacterium (Withdrawn 2014)
DIN EN ISO 16712:2007-02 Water quality - Determination of acute toxicity of marine or estuarine sediment to amphipods (ISO 16712:2005)
EN ISO 16712:2006 Water quality - Determination of acute toxicity of marine or estuarine sediment to amphipods (ISO 16712:2005)
ASTM E 1242 : 1997 : R2003 Standard Practice for Using Octanol-Water Partition Coefficient to Estimate Median Lethal Concentrations for Fish Due to Narcosis
ASTM E 1218 : 2004 Standard Guide for Conducting Static Toxicity Tests with Microalgae
BS ISO 16712 : 2005 AMD 16801 WATER QUALITY - DETERMINATION OF ACUTE TOXICITY OF MARINE OR ESTUARINE SEDIMENT TO AMPHIPODS
BS 6068-6.16(1998) : 1998 WATER QUALITY - SAMPLING - GUIDANCE ON BIOTESTING OF SAMPLES
ASTM D 6081 : 1998 : R2004 Standard Practice for Aquatic Toxicity Testing of Lubricants: Sample Preparation and Results Interpretation
FM 5138 : 2011 PROPORTIONING TESTING
ASTM E 1192 : 1997 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1191 : 2003 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
ASTM E 1913 : 2004 : R2012 <i xmlns:a="http://library.astm.org" xmlns="">Myriophyllum sibiricum</i>
ASTM E 1463 : 1992 : R2012 Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States (Withdrawn 2021)
ASTM E 1242 : 1997 : R2014 Standard Practice for Using Octanol-Water Partition Coefficient to Estimate Median Lethal Concentrations for Fish Due to Narcosis
ASTM E 1562 : 2000 : R2006 Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids
ASTM E 1191 : 2003 : REV A : R2008 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
ASTM E 1624 : 1994 Standard Guide for Chemical Fate in Site-Specific Sediment/Water Microcosms
ISO 16712:2005 Water quality — Determination of acute toxicity of marine or estuarine sediment to amphipods
ASTM E 1624 : 1994 : R2002 Standard Guide for Chemical Fate in Site-Specific Sediment/Water Microcosms
ASTM D 5951 : 1996 : R2002 Standard Practice for Preparing Residual Solids Obtained After Biodegradability Standard Methods for Plastics in Solid Waste for Toxicity and Compost Quality Testing (Withdrawn 2011)
16/30282355 DC : 0 BS ISO 35103 - PETROLEUM AND NATURAL GAS INDUSTRIES - ARCTIC OPERATIONS - ENVIRONMENTAL MONITORING
ASTM E 1193:1997 Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
BS 6068-5.37(2005) : 2005 WATER QUALITY - DETERMINATION OF ACUTE TOXICITY OF MARINE OR ESTUARINE SEDIMENT TO AMPHIPODS
ASTM E 1218 : 2004 : EDT 1 Standard Guide for Conducting Static Toxicity Tests with Microalgae
ASTM D 6081 : 1998 : R2009 Standard Practice for Aquatic Toxicity Testing of Lubricants: Sample Preparation and Results Interpretation
ASTM E 1924 : 1997 : R2012 Standard Guide for Conducting Toxicity Tests with Bioluminescent Dinoflagellates (Withdrawn 2013)
BS ISO 35103:2017 Petroleum and natural gas industries. Arctic operations. Environmental monitoring
ASTM D 3696 : 2000 Standard Practice for Evaluating an Effluent for Flavor Impairment to Fish Flesh (Withdrawn 2006)
ASTM E 1498 : 1992 : R2004 Standard Guide for Conducting Sexual Reproduction Tests with Seaweeds
ASTM E 1192 : 1997 : R2008 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1768 : 1995 : R2008 Standard Guide for Ventilatory Behavioral Toxicology Testing of Freshwater Fish
ASTM E 1525 : 2002 Standard Guide for Designing Biological Tests with Sediments
ASTM E 1841 : 2004 Standard Guide for Conducting Renewal Phytotoxicity Tests With Freshwater Emergent Macrophytes
ASTM E 1463 : 1992 : R1998 Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States
ASTM E 1688 : 2010 : R2016 Standard Guide for Determination of the Bioaccumulation of Sediment-Associated Contaminants by Benthic Invertebrates
ASTM E 1391 : 2003 : R2008 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 1192 : 1997 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1022 : 1994 : R2007 Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks
ASTM E 1295 : 2001 : R2013 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <emph type="ital">Ceriodaphnia dubia</emph><emph type="bold">
ASTM E 2122 : 2002 : R2007 Standard Guide for Conducting In-situ Field Bioassays With Caged Bivalves
ASTM E 2455 : 2006 : R2013 Standard Guide for Conducting Laboratory Toxicity Tests with Freshwater Mussels (Withdrawn 2022)
ASTM E 1241 : 2005 : R2013 Standard Guide for<brk type="line"/> Conducting Early Life-Stage Toxicity Tests with Fishes (Withdrawn 2022)
BS EN ISO 16712:2006 Water quality. Determination of acute toxicity of marine or estuarine sediment to amphipods

ASTM E 1604 : 2012 : REDLINE Standard Guide for Behavioral Testing in Aquatic Toxicology
IEEE/ASTM SI_10-2010 American National Standard for Metric Practice

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