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ASTM E 1563 : 1998 : R2012

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos
Available format(s)

Hardcopy , PDF

Superseded date

02-11-2021

Language(s)

English

Published date

01-09-2013

CONTAINED IN VOL. 11.06, 2015 Explains procedures for obtaining laboratory data concerning the acute effects of a test material on embryos and the resulting larvae of echinoid embryos (sea urchins and sand dollars) during static 48- to 96-h exposures.

1.1This guide covers procedures for obtaining laboratory data concerning the acute effects of a test material on embryos and the resulting larvae of echinoid embryos (sea urchins and sand dollars) during static 48- to 96-h exposures. These procedures have generally been used with U.S. East Coast (Arbacia punctulata and Strongylocentrotus droebachiensis ) (1)3 and West Coast species (Strongylocentrotus purpuratus, S. droebachiensis, and Dendraster excentricus) (2). The basic procedures described in this guide first originated in Japan and Scandanavia (3), and parallel procedures have been used with foreign species, especially in Japan and the Mediterranean (4). These procedures will probably be useful for conducting static acute toxicity tests with embryos of other echinoid species, although modifications might be necessary.

1.2Other modifications of these procedures might be justified by special needs or circumstances. Although using procedures appropriate to a particular species or special needs and circumstances is more important than following prescribed procedures, the results of tests conducted by using unusual procedures are not likely to be comparable with those of many other tests. The comparison of results obtained by using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute tests starting with embryos of echinoids.

1.3These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct acute tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, surface waters and sediments (Annex A1). Renewal tests might be preferable to static tests for materials that have a high oxygen demand, are highly volatile, are rapidly transformed biologically or chemically in aqueous solution, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test.

1.4Results of acute toxicity tests with echinoid embryos should usually be reported as the 50 % effect concentration (EC50) based on the total abnormally developed embryos and larvae. In some situations, it might only be necessary to determine whether a specific concentration is acutely toxic to embryos or whether the EC50 is above or below a specific concentration.

1.5This guide is arranged as follows:


Section

Scope

1

Referenced Documents

2

Terminology

3

Summary of Guide

4

Significance and Use

5

Apparatus

6

 Facilities

6.1

 Construction Materials

6.2

 Test Chambers

6.3

 Cleaning

6.4

 Acceptability

6.5

Safety Precautions

7

Dilution Water

8

 Requirements

8.1

 Source

8.2

 Treatment

8.3

 Characterization

8.4

Test Material

9

 General

9.1

 Stock Solution

9.2

 Test Concentration(s)

9.3

Test Organisms

10

 Species

10.1

 Age

10.2

 Source of Embryos

10.3

 Handling

10.4

 Test Animal Source and Condition

10.5

 Spawning and Fertilization

10.6

 Quality

10.7

Procedure

11

 Experimental Design

11.1

 Dissolved Oxygen

11.2

 Temperature

11.3

 Beginning the Test

11.4

 Feeding

11.5

 Duration of Test

11.6

 Biological Data

11.7

 Control Performance

11.8

 Other Measurements

11.9

Analytical Methods

12

Acceptability of Test

13

Calculation of Results

14

Report

15

Keywords

16

Annex

 Sediment Tests

Annex A1




1.6The values stated in SI units are to be regarded as the standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations. Specific precautionary statements are given in Section 7.

Committee
E 50
DocumentType
Guide
Pages
22
ProductNote
Reconfirmed 2012
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

ASTM E 1525 : 2002 : R2014 Standard Guide for Designing Biological Tests with Sediments
ASTM E 729 : 1996 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests

ASTM E 1367 : 2003 : EDT 1 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1023 : 1984 : R2007 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1391 : 2002 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing
ASTM E 1706 : 2005 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 380 : 1993 Standard Practice for Use of the Internation System of Units (SI) (the Modernized Metric System) (Withdrawn 1997)
ASTM E 1391 : 2003 : R2014 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 1525 : 1994 : REV A Standard Guide for Designing Biological Tests with Sediments
ASTM E 1023 : 1984 : R2014 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1367 : 2003 : R2014 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1023 : 1984 : R2002 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1706 : 2000 : EDT 1 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Fresh Water Invertebrates
ASTM E 1706 : 2020 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 1391 : 1994 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing
ASTM E 1391 : 2003 : R2008 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 1706 : 2000 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Fresh Water Invertebrates
ASTM E 1706 : 2005 : EDT 1 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 1192 : 1997 : R2008 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1367 : 2003 : R2008 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1023 : 1984 : R1996 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1367 : 1992 Standard Guide for Conducting 10-day Static Sediment Toxicity Tests with Marine and Estuarine Amphipods
ASTM E 1391 : 2003 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 1192 : 1997 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1367 : 2003 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1706 : 2005 : R2010 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates (Withdrawn 2019)
ASTM E 1525 : 2002 : R2008 Standard Guide for Designing Biological Tests with Sediments
ASTM E 1706 : 2000 : EDT 2 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 1192 : 1997 : R2003 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1706 : 2004 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1192 : 1997 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1706 : 2019 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 1525 : 2002 Standard Guide for Designing Biological Tests with Sediments
ASTM E 1367 : 1999 Standard Guide for Conducting 10-day Static Sediment Toxicity Tests with Marine and Estuarine Amphipods
ASTM E 1525 : 2002 : R2014 Standard Guide for Designing Biological Tests with Sediments

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