Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Requirements for sample preparation for qualitative detection

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

28-04-2006

Publisher

British Standards Institution

This International Standard provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.

It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B.

This International Standard has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.

Committee	AW/9
DevelopmentNote	Supersedes 04/30114070 DC. (04/2006)
DocumentType	Standard
Pages	18
PublisherName	British Standards Institution
Status	Current
Supersedes	04/30114070 DC : DRAFT MAY 2004

Standards	Relationship
EN ISO 20837:2006	Identical
SN EN ISO 20837 : 2006	Identical
ISO 20837:2006	Identical
NS EN ISO 20837 : 1ED 2006	Identical
UNI EN ISO 20837 : 2006	Identical
NBN EN ISO 20837 : 2006	Identical
UNE-EN ISO 20837:2007	Identical
I.S. EN ISO 20837:2006	Identical
NF EN ISO 20837 : 2006	Identical
NEN EN ISO 20837 : 2006	Identical
DIN EN ISO 20837:2006-08	Identical
DIN EN ISO 20837 E : 2006	Identical

ISO 22174:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
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ISO 21567:2004	Microbiology of food and animal feeding stuffs Horizontal method for the detection of Shigella spp.
ISO 11290-1:2017	Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 1: Detection method
ISO/TS 20836:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance testing for thermal cyclers
ISO 7937:2004	Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of Clostridium perfringens — Colony-count technique
ISO 10272-1:2017	Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method
ISO 6888-3:2003	Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part 3: Detection and MPN technique for low numbers
ISO 7932:2004	Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of presumptive Bacillus cereus Colony-count technique at 30 degrees C
ISO 10273:2017	Microbiology of the food chain — Horizontal method for the detection of pathogenic Yersinia enterocolitica
ISO 20838:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 16654:2001	Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157
DIN 10135:2013-05	MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - METHOD FOR THE DETECTION OF SALMONELLA