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EN ISO 22160:2007

Current

Current

The latest, up-to-date edition.

Milk and milk-based drinks - Determination of alkaline phosphatase activity - Enzymatic photo-activated system (EPAS) method (ISO 22160:2007)

Published date

01-04-2007

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Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparations
   7.1 Test samples
   7.2 Alkaline phosphatase-free milk
8 Procedure
   8.1 Calibration
   8.2 Calibration adjustment
   8.3 Control tests and calibration check
   8.4 Determination
   8.5 Heat-resistant microbial alkaline phosphatase
        control
9 Calculation and expression of results
   9.1 General
   9.2 Manual calculation
   9.3 Expression of results
10 Precision
   10.1 Interlaboratory test
   10.2 Repeatability
   10.3 Reproducibility
11 Test report
Annex A (informative) Procedure flowchart
Annex B (informative) Results of interlaboratory
        trials
Bibliography

ISO 22160|IDF 209:2007 specifies a method for the determination of the alkaline phosphatase activity in pasteurized whole milk, semi-skimmed milk, skimmed milk, cream and flavoured milks using a chemiluminescent (EPAS) method.The method is applicable to milk and milk-based drinks from cows, sheep, buffalo and goats. The method is also suitable for any liquid-based sample if diluted in such a way that the diluted alkaline phosphatase activity has less than 7 000 milliunits per litre.

Committee
CEN/TC 302
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 11816-1:2013 Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks
ISO 11816-2:2016 Milk and milk products — Determination of alkaline phosphatase activity — Part 2: Fluorimetric method for cheese
ISO 707:2008 Milk and milk products — Guidance on sampling
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results — Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method

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